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KMID : 0380020170320040328
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Korean Journal of Biotechnology and Bioengineering
2017 Volume.32 No. 4 p.328 ~ p.334
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Inhibitory Activity of Ligustrum japonicum Fructus on MMP-2 and MMP-9
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Kim Ho-Jun
Kong Chang-suk
Seo Young-Wan
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Abstract
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In this study, Ligustrum japonicum fructus was studied for its ability to inhibit MMP-2 and -9 using gelatin zymography and RT-PCR in Phorbol 12-myristate 13-acetate (PMA)-induced HT1080 fibrosarcoma cells. Ligustrum japonicum fructus was extracted twice with dichloromethane (CH2Cl2) and methanol (MeOH) in turn. The combined crude extracts (CH2Cl2 and MeOH) of Ligustrum japonicum fructus showed a significant inhibitory effect on MMP-2 and -9 activities in gelatin zymography. The combined crude extracts were partitioned between CH2Cl2 and water. The organic layer was further partitioned between 85% aq.MeOH and n-hexane, and then the aqueous layer was fractionated with n-butanol (n-BuOH) and water, successively. Among all solvent fractions, n-BuOH and 85% aq.MeOH fractions showed the potent inhibitory effect on MMP-2 and -9 in gelatin zymography. In PMA-treated HT-1080 cells, mRNA expression of MMP- 2/-9 was also greatly inhibited in the n-BuOH and 85% aq.MeOH fractions. In conclusion, these findings suggested that Ligustrum japonicum fructus can be used as a potential source of promising anti-invasive agent.
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KEYWORD
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Ligustrum japonicum fructus, HT-1080, anti-invasive, MMP inhibition
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